PrimePCR™ Probe Assay: PHS1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PHS1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PHS1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0025737
Assay Design:   Exonic
Chromosome Location:   3:17459356-17459474question
Amplicon Length:   89
Splice Variants Targeted:   AT5G65410.1 AT3G47390.1 AT3G47390.2

Gene Information

Encodes a protein that is believed to function as a pyrimidine reductase involved in riboflavin and FAD biosynthesis. phs1 was identified as a photosensitive mutant that shows reduced growth, chloroplast developmental abnormalities, reduced chlorophyll levels, increased oxidative stress, reduced NADPH/NADP+ ratios, reduced photosystem I electron transport, and reduced photosynthetic protein levels under high light conditions. Many of these abnormal phenotypes likely arise from the reduction in the levels of FAD in the phs1 mutant.

Gene Symbol:   PHS1
Gene Name:   Cytidine/deoxycytidylate deaminase family protein
Aliases:   Not Available
RefSeq:   NM_001203097 NM_114607
Ensembl:   AT3G47390
Entrez:   823893
Chromosome Mapping:   Not Available

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