PrimePCR™ Probe Assay: 4CL1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: 4CL1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: 4CL1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0023649
Assay Design:   Exonic
Chromosome Location:   1:19160794-19160930question
Amplicon Length:   107
Splice Variants Targeted:   AT3G21240.1 AT1G51680.1 AT1G51680.2 AT1G51680.3

Gene Information

encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. In addition to 4-coumarate, it also converts ferulate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, ferulic acid, caffeic acid and 5-OH-ferulic acid. At4CL1 was unable to use sinapic acid as substrate.

Gene Symbol:   4CL1
Gene Name:   4-coumarate:CoA ligase 1
Aliases:   Not Available
RefSeq:   NM_104046 NM_001084228 NM_179462
Ensembl:   AT1G51680
Entrez:   841593
Chromosome Mapping:   Not Available

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