PrimePCR™ Probe Assay: BES1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BES1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BES1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0023204
Assay Design:   Exonic
Chromosome Location:   1:6689435-6689565question
Amplicon Length:   101
Splice Variants Targeted:   AT3G01320.1 AT3G01320.2 AT1G19350.1 AT1G19350.3 AT1G19350.4 AT1G19350.5 AT1G19350.6

Gene Information

Encodes brassinosteroid (BR) signalling protein that accumulates in the nucleus as dephosphorylated form in response to BRs. Is phosphorylated by the BIN2 GSK3 kinase. It synergistically interacts with BIM1 to bind to E box sequences (CANNTG). The protein contains a nuclear localization signal (NLS), followed by a highly conserved amino-terminal domain (N) shared by all family members, a BIN2 phosphorylation domain (P), a PEST motif, involved in protein degradation in the absence of BR, and a carboxyl-terminal domain. BES1 can interact with the ELF6 and REF6 Jumonji N/C-domain containing proteins and may direct them to modify histone methylation upstream of some brassinosteroid responsive-genes

Gene Symbol:   BES1
Gene Name:   Brassinosteroid signalling positive regulator (BZR1) family protein
Aliases:   Not Available
RefSeq:   NM_202135 NM_202134 NM_202136 NM_101792 NM_001084093
Ensembl:   AT1G19350
Entrez:   838518
Chromosome Mapping:   Not Available

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