PrimePCR™ Probe Assay: CER1, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CER1, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CER1, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0023040
Assay Design:   Exonic
Chromosome Location:   1:422056-422245question
Amplicon Length:   160
Splice Variants Targeted:   AT2G02570.1 AT2G02570.2 AT2G02570.3 AT2G02570.4 AT1G02205.1 AT1G02205.2 AT1G02205.3

Gene Information

Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.

Gene Symbol:   CER1
Gene Name:   Fatty acid hydroxylase superfamily
Aliases:   Not Available
RefSeq:   NM_001197961 NM_100101 NM_180601
Ensembl:   AT1G02205
Entrez:   837602
Chromosome Mapping:   Not Available

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