PrimePCR™ Probe Assay: MEP1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MEP1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MEP1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029834
Assay Design:   Intron-spanning
Chromosome Location:   18:29793465-29795091question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000269202

Gene Information

Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted whereas oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability renal gene expression profiles and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq Oct 2011]

Gene Symbol:   MEP1B
Gene Name:   meprin A, beta
Aliases:   Not Available
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000141434
Entrez:   4225
Chromosome Mapping:   18q12.2-q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.350000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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