This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028609
PrimePCR™ PreAmp for SYBR® Green Assay: TTR, Human
PrimePCR™ Template for SYBR® Green Assay: TTR, Human
This gene encodes transthyretin one of the three prealbumins including alpha-1-antitrypsin transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition affecting predominantly peripheral nerve and/or the heart and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy euthyroid hyperthyroxinaemia amyloidotic vitreous opacities cardiomyopathy oculoleptomeningeal amyloidosis meningocerebrovascular amyloidosis carpal tunnel syndrome etc. [provided by RefSeq Jan 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.