PrimePCR™ SYBR® Green Assay: INPP5J, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0023941

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: INPP5J, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: INPP5J, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001090
Assay Design:   Exonic
Chromosome Location:   22:31524457-31524616question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000331075 ENST00000400294 ENST00000405300 ENST00000404390 ENST00000402238 ENST00000404453 ENST00000401755 ENST00000412277

Gene Information

Description Not Available

Gene Symbol:   INPP5J
Gene Name:   inositol polyphosphate-5-phosphatase J
Aliases:   INPP5, MGC129984, PIB5PA, PIPP
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000185133
Entrez:   27124
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.180000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download