PrimePCR™ SYBR® Green Assay: Fbxl20, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0051388
Assay Design:   Intron-spanning
Chromosome Location:   10:85848953-85850459question
Amplicon Length:   75
Splice Variants Targeted:   ENSRNOT00000067317

Gene Information

F-box protein homologous to Skp2 that interacts with Skp1 [RGD, Feb 2006]

Gene Symbol:   Fbxl20
Gene Name:   F-box/LRR-repeat protein 20
Aliases:   Not Available
RefSeq:   NM_022272
Ensembl:   ENSRNOG00000005081
Entrez:   64039
UniGene:   Rn.11637,Rn.18281
Chromosome Mapping:   10q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.760000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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