This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Adam22, Rat
PrimePCR™ Template for SYBR® Green Assay: Adam22, Rat
metalloprotease-disintegrin containing a catalytic zinc ion with moderate sequence similarity to human metalloproteinase-disintegrin domain 22 (MDC2 delta), isoform 1 preproprotein [RGD, Feb 2006]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.