PrimePCR™ SYBR® Green Assay: Adam22, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0010792
Assay Design:   intron_spanning
Chromosome Location:   4:22822881-22825201question
Amplicon Length:   181
Splice Variants Targeted:   ENSRNOT00000073595 ENSRNOT00000070872 ENSRNOT00000071921 ENSRNOT00000072068 ENSRNOT00000073723 ENSRNOT00000073501 ENSRNOT00000074918 ENSRNOT00000073044 ENSRNOT00000074832 ENSRNOT00000068410 ENSRNOT00000071635

Gene Information

metalloprotease-disintegrin containing a catalytic zinc ion with moderate sequence similarity to human metalloproteinase-disintegrin domain 22 (MDC2 delta), isoform 1 preproprotein [RGD, Feb 2006]

Gene Symbol:   Adam22
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000042478
Entrez:   57033
UniGene:   Rn.158744
Chromosome Mapping:   4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.190000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download