PrimePCR™ SYBR® Green Assay: Erbb3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0001330
Assay Design:   intron_spanning
Chromosome Location:   7:2971655-2972554question
Amplicon Length:   104
Splice Variants Targeted:   ENSRNOT00000006796

Gene Information

acts as a tyrosine kinase receptor for neuregulin; may play a role in axonal regeneration in response to injury [RGD, Feb 2006]

Gene Symbol:   Erbb3
Gene Name:   Receptor tyrosine-protein kinase erbB-3 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000004964
Entrez:   29496
UniGene:   Rn.10228
Chromosome Mapping:   7q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.190000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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