PrimePCR™ SYBR® Green Assay: RGD1560194, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0056759
Assay Design:   Exonic
Chromosome Location:   3:10223546-10223671question
Amplicon Length:   96
Splice Variants Targeted:   ENSRNOT00000037123 ENSRNOT00000006485 ENSRNOT00000044593 ENSRNOT00000009816 ENSRNOT00000043418 ENSRNOT00000049436 ENSRNOT00000006754 ENSRNOT00000044551 ENSRNOT00000014590 ENSRNOT00000073389 ENSRNOT00000045509 ENSRNOT00000013435 ENSRNOT00000034377 ENSRNOT00000064857 ENSRNOT00000019331 ENSRNOT00000071898 ENSRNOT00000002935 ENSRNOT00000073752 ENSRNOT00000070922 ENSRNOT00000041103 ENSRNOT00000031545 ENSRNOT00000046365 ENSRNOT00000047694 ENSRNOT00000039882 ENSRNOT00000047511 ENSRNOT00000047713 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1560194
Gene Name:   60S ribosomal protein L7a
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049097
Entrez:   366784
UniGene:   Not Available
Chromosome Mapping:   7q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.400000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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