PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000046854, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000046854, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000046854, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0020381
Assay Design:   exonic
Chromosome Location:   4:167541003-167541094question
Amplicon Length:   62
Splice Variants Targeted:   ENSRNOT00000042220 ENSRNOT00000066676 ENSRNOT00000049969 ENSRNOT00000074237 ENSRNOT00000073407 ENSRNOT00000042300 ENSRNOT00000048461 ENSRNOT00000048400 ENSRNOT00000043961 ENSRNOT00000051084 ENSRNOT00000050573 ENSRNOT00000051723 ENSRNOT00000075229

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000046854
Gene Name:   Protein LOC100362150
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046854
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.380000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download