PrimePCR™ SYBR® Green Assay: St8sia1, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0003166
Assay Design:   exonic
Chromosome Location:   4:241274314-241274438question
Amplicon Length:   95
Splice Variants Targeted:   ENSRNOT00000018999

Gene Information

an alpha-2,8-sialyltransferase that catalyzes the biosynthesis of GT1a and GQ1b as well as GD3 [RGD, Feb 2006]

Gene Symbol:   St8sia1
Gene Name:   Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
Aliases:   Not Available
RefSeq:   NM_012813
Ensembl:   ENSRNOG00000014018
Entrez:   25280
UniGene:   Rn.96242
Chromosome Mapping:   4q44

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.170000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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