PrimePCR™ SYBR® Green Assay: Rnase17, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Rnase17, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rnase17, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0003099
Assay Design:   exonic
Chromosome Location:   15:32034434-32034609question
Amplicon Length:   146
Splice Variants Targeted:   ENSRNOT00000022637 ENSRNOT00000045533 ENSRNOT00000040857 ENSRNOT00000041413 ENSRNOT00000025273 ENSRNOT00000006162 ENSRNOT00000025978 ENSRNOT00000037523 ENSRNOT00000009872 ENSRNOT00000064211 ENSRNOT00000003006 ENSRNOT00000060465 ENSRNOT00000018695 ENSRNOT00000028331 ENSRNOT00000023137 ENSRNOT00000060987 ENSRNOT00000071158 ENSRNOT00000048941 ENSRNOT00000071093

Gene Information

Description Not Available

Gene Symbol:   Rnase17
Gene Name:   Ribonuclease 17 precursor
Aliases:   Not Available
RefSeq:   NM_001012232
Ensembl:   ENSRNOG00000050295
Entrez:   497195
Chromosome Mapping:   15p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.270000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download