PrimePCR™ SYBR® Green Assay: Gzmd, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0043286

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gzmd, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gzmd, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0041069
Assay Design:   Intron-spanning
Chromosome Location:   14:56130758-56132498question
Amplicon Length:   189
Splice Variants Targeted:   ENSMUST00000178228 ENSMUST00000059699 ENSMUST00000069098 ENSMUST00000070552 ENSMUST00000079510 ENSMUST00000009390 ENSMUST00000097542 ENSMUST00000169849 ENSMUST00000021832 ENSMUST00000059428 ENSMUST00000019248 ENSMUST00000108125 ENSMUST00000145910 ENSMUST00000024881 ENSMUST00000021458 ENSMUST00000166101 ENSMUST00000059341 ENSMUST00000036387 ENSMUST00000074660 ENSMUST00000112878 ENSMUST00000166406 ENSMUST00000021420 ENSMUST00000110177 ENSMUST00000098457 ENSMUST00000150503 ENSMUST00000020528 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Gzmd
Gene Name:   Granzyme D
Aliases:   CCP5, Ctla-5, Ctla5
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000059256
Entrez:   14941
Chromosome Mapping:   14 C3|14 20.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 39.870000
Efficiency 85

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download