PrimePCR™ SYBR® Green Assay: Kcnk3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Kcnk3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Kcnk3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020939
Assay Design:   Intron-spanning
Chromosome Location:   5:30588567-30622004question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000086101 ENSMUST00000144408 ENSMUST00000089752 ENSMUST00000149975 ENSMUST00000022337 ENSMUST00000079362 ENSMUST00000115781 ENSMUST00000055242 ENSMUST00000098068 ENSMUST00000106468 ENSMUST00000004145 ENSMUST00000107356 ENSMUST00000107357 ENSMUST00000045840 ENSMUST00000044624 ENSMUST00000093193 ENSMUST00000101365 ENSMUST00000060989 ENSMUST00000090591 ENSMUST00000113599 ENSMUST00000108849 ENSMUST00000020830 ENSMUST00000032168 ENSMUST00000170909 ENSMUST00000110888 ENSMUST00000048371 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Kcnk3
Gene Name:   Potassium channel, subfamily K, member 3
Aliases:   TASK, cTBAK-1
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000049265
Entrez:   16527
Chromosome Mapping:   5 B|5 18.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.440000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download