PrimePCR™ SYBR® Green Assay: Chst13, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Chst13, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Chst13, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020771
Assay Design:   Intron-spanning
Chromosome Location:   6:90318282-90325185question
Amplicon Length:   141
Splice Variants Targeted:   ENSMUST00000119567 ENSMUST00000159525 ENSMUST00000100688 ENSMUST00000166743 ENSMUST00000164235 ENSMUST00000120194 ENSMUST00000097542 ENSMUST00000006123 ENSMUST00000107279 ENSMUST00000107277 ENSMUST00000153965 ENSMUST00000035121 ENSMUST00000130602 ENSMUST00000133533 ENSMUST00000105644 ENSMUST00000020322 ENSMUST00000081688 ENSMUST00000172694 ENSMUST00000173495 ENSMUST00000108270 ENSMUST00000108271 ENSMUST00000070890 ENSMUST00000161600 ENSMUST00000097724 ENSMUST00000038372 ENSMUST00000042221 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Chst13
Gene Name:   Carbohydrate (chondroitin 4) sulfotransferase 13
Aliases:   1110067M19Rik, C4ST-3
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000056643
Entrez:   71797
Chromosome Mapping:   6 D1|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.330000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download