PrimePCR™ SYBR® Green Assay: Orai2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020767
Assay Design:   Intron-spanning
Chromosome Location:   5:136150820-136161580question
Amplicon Length:   197
Splice Variants Targeted:   ENSMUST00000078108 ENSMUST00000165834 ENSMUST00000049271 ENSMUST00000003964 ENSMUST00000107766 ENSMUST00000038720 ENSMUST00000035515 ENSMUST00000002044 ENSMUST00000115642 ENSMUST00000145675 ENSMUST00000033501 ENSMUST00000102484 ENSMUST00000177592 ENSMUST00000113672 ENSMUST00000081636 ENSMUST00000103178 ENSMUST00000030922 ENSMUST00000123652 ENSMUST00000131975 ENSMUST00000114689 ENSMUST00000002095 ENSMUST00000118828 ENSMUST00000122103 ENSMUST00000120691 ENSMUST00000072363 ENSMUST00000046371 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Orai2
Gene Name:   ORAI calcium release-activated calcium modulator 2
Aliases:   A730041O15Rik, Tmem142b
RefSeq:   NC_000071.6 NT_039314.8
Ensembl:   ENSMUSG00000039747
Entrez:   269717
UniGene:   Mm.308396
Chromosome Mapping:   5 G2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.050000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download