This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Musk, Mouse
PrimePCR™ Template for SYBR® Green Assay: Musk, Mouse
This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development including organization of the postsynaptic membrane synaptic gene transcription patterning of skeletal muscle anchoring of acetylcholinesterase and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.