PrimePCR™ SYBR® Green Assay: Meis2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0030297

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0014078
Assay Design:   Intron-spanning
Chromosome Location:   2:116059519-116061500question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000110908 ENSMUST00000110907 ENSMUST00000028639 ENSMUST00000102538 ENSMUST00000110906 ENSMUST00000074285 ENSMUST00000134314 ENSMUST00000133990

Gene Information

Description Not Available

Gene Symbol:   Meis2
Gene Name:   Meis homeobox 2
Aliases:   A430109D20Rik, Mrg1, Stra10
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000027210
Entrez:   17536
UniGene:   Mm.247566
Chromosome Mapping:   2 E4-E5|2 64.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.920000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download