This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0030845
PrimePCR™ PreAmp for SYBR® Green Assay: Acadm, Mouse
PrimePCR™ Template for SYBR® Green Assay: Acadm, Mouse
This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple intronless pseudogenes. [provided by RefSeq Nov 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.