PrimePCR™ SYBR® Green Assay: Lmo2, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0030616

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0011802
Assay Design:   Intron-spanning
Chromosome Location:   2:103970666-103980965question
Amplicon Length:   108
Splice Variants Targeted:   ENSMUST00000156813 ENSMUST00000123437 ENSMUST00000111143 ENSMUST00000111140 ENSMUST00000111139 ENSMUST00000170926 ENSMUST00000163256

Gene Information

Description Not Available

Gene Symbol:   Lmo2
Gene Name:   LIM domain only 2
Aliases:   Rbtn-2, Rbtn2, Rhom-2, Ttg2
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000032698
Entrez:   16909
UniGene:   Mm.29266
Chromosome Mapping:   2 E2|2 60.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.500000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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