This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0028553
PrimePCR™ PreAmp for SYBR® Green Assay: Rrp15, Mouse
PrimePCR™ Template for SYBR® Green Assay: Rrp15, Mouse
This gene encodes a protein similar to budding yeast Rrp15p. Rrp15p is a component of pre-60S ribosomal particles in budding yeast and is required for the early maturation steps of the 60S subunit. The mouse genome contains at least one pseudogene on the X chromosome. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.