This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0029238
PrimePCR™ PreAmp for SYBR® Green Assay: Nppc, Mouse
PrimePCR™ Template for SYBR® Green Assay: Nppc, Mouse
This gene encodes a member of the natriuretic peptide family. Natriuretic peptides are involved in the control of blood pressure extracellular fluid volume and electrolyte homeostasis. The encoded protein also plays a role in sensory neuron bifurcation and is a critical regulator of endochondral bone growth. The encoded protein is a ligand for the natriuretic peptide receptor B and is synthesized as a preprohormone which is cleaved to produce a mature peptide. Mutations in this gene are associated with dwarfism resulting from impaired endochondral ossification. [provided by RefSeq Apr 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.