PrimePCR™ SYBR® Green Assay: Smok3a, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062834

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061685
Assay Design:   exonic
Chromosome Location:   5:138032557-138032655question
Amplicon Length:   69
Splice Variants Targeted:   ENSMUST00000080647 ENSMUST00000162940 ENSMUST00000059824 ENSMUST00000074512 ENSMUST00000144015 ENSMUST00000115649 ENSMUST00000085886 ENSMUST00000171498 ENSMUST00000100537 ENSMUST00000166206

Gene Information

Description Not Available

Gene Symbol:   Smok3a
Gene Name:   Sperm motility kinase 3A
Aliases:   Smok2b1
RefSeq:   NC_000071.6 NT_039315.8
Ensembl:   ENSMUSG00000075599
Entrez:   545814
UniGene:   Mm.349005
Chromosome Mapping:   5 G2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.100000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download