PrimePCR™ SYBR® Green Assay: Foxn3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062578

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061429
Assay Design:   exonic
Chromosome Location:   12:99388709-99388825question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000027438 ENSMUST00000090339 ENSMUST00000169385 ENSMUST00000113658 ENSMUST00000032057 ENSMUST00000108689 ENSMUST00000165221 ENSMUST00000007301 ENSMUST00000046859 ENSMUST00000177451 ENSMUST00000177269 ENSMUST00000176928 ENSMUST00000085108

Gene Information

Description Not Available

Gene Symbol:   Foxn3
Gene Name:   Forkhead box N3
Aliases:   5430426H20Rik, AA589593, AW556347, Ches1, Ches1l, HTLFL1
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000033713
Entrez:   71375
UniGene:   Mm.341972
Chromosome Mapping:   12 E|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.690000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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