PrimePCR™ SYBR® Green Assay: Vmn1r34, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0060679

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052255
Assay Design:   exonic
Chromosome Location:   6:66637463-66637598question
Amplicon Length:   106
Splice Variants Targeted:   ENSMUST00000176838 ENSMUST00000096612 ENSMUST00000164307 ENSMUST00000079669 ENSMUST00000171186 ENSMUST00000176073 ENSMUST00000176376 ENSMUST00000177267 ENSMUST00000049694 ENSMUST00000168416 ENSMUST00000079584 ENSMUST00000074381 ENSMUST00000071414 ENSMUST00000177072 ENSMUST00000077482

Gene Information

Description Not Available

Gene Symbol:   Vmn1r34
Gene Name:   Vomeronasal 1 receptor 34
Aliases:   EG546901, Gm5991
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000091012
Entrez:   546901
UniGene:   Mm.480276
Chromosome Mapping:   6 C1|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.980000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download