PrimePCR™ SYBR® Green Assay: Prdx6b, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0059049

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0050623
Assay Design:   exonic
Chromosome Location:   2:80295137-80295273question
Amplicon Length:   107
Splice Variants Targeted:   ENSMUST00000057072

Gene Information

This intronless gene is similar to the multi-exon peroxiredoxin 6 gene located on chromosome 1. It is transcribed and the ORF is intact compared to that of the peroxiredoxin 6 gene. This gene could be considered a transcribed pseudogene based on a failure to detect the protein in vivo in PMID:14644414. However NCBI is representing the protein due to mass spectrometry data in PMID:18614015 which detected at least one peptide that is specific for this protein. [provided by RefSeq Mar 2011]

Gene Symbol:   Prdx6b
Gene Name:   Peroxiredoxin 6B
Aliases:   1-cysPrx-P1, 4930414C22Rik, Aop2-rs1, Prdx5-rs1, Prdx6-ps1, Prdx6-rs1, Prdx6-rs1-ps
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000050114
Entrez:   320769
UniGene:   Mm.60992
Chromosome Mapping:   2 C3|2 47.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.180000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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