PrimePCR™ SYBR® Green Assay: Arhgef2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0055298

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046872
Assay Design:   exonic
Chromosome Location:   3:88629790-88629922question
Amplicon Length:   103
Splice Variants Targeted:   ENSMUST00000175903 ENSMUST00000177023 ENSMUST00000176539 ENSMUST00000177498 ENSMUST00000176500 ENSMUST00000175779 ENSMUST00000029694 ENSMUST00000176804 ENSMUST00000107510 ENSMUST00000170653 ENSMUST00000177303 ENSMUST00000176307 ENSMUST00000175911 ENSMUST00000176243 ENSMUST00000176316 ENSMUST00000176879 ENSMUST00000175745

Gene Information

Description Not Available

Gene Symbol:   Arhgef2
Gene Name:   Rho/rac guanine nucleotide exchange factor (GEF) 2
Aliases:   AA408978, GEF, GEF-H1, GEFH1, LFP40, Lbcl1, Lfc, P40, mKIAA0651
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000028059
Entrez:   16800
UniGene:   Mm.239329
Chromosome Mapping:   3 F1|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.310000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download