This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCEP0053626
PrimePCR™ PreAmp for SYBR® Green Assay: Tead3, Mouse
PrimePCR™ Template for SYBR® Green Assay: Tead3, Mouse
This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors which contain the TEA/ATTS DNA-binding domain. It is predominantly expressed in the placenta and thought to play a role in placental gene regulation and development. Alternative splicing and alternate use of an upstream AUG translation initiation codon and an in-frame downstream non-AUG (AUA) codon results in 2 isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.