PrimePCR™ SYBR® Green Assay: Nudt1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0044026

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Nudt1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Nudt1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041810
Assay Design:   exonic
Chromosome Location:   5:140337645-140337829question
Amplicon Length:   155
Splice Variants Targeted:   ENSMUST00000003574 ENSMUST00000094821 ENSMUST00000128570 ENSMUST00000162849 ENSMUST00000037232 ENSMUST00000178977 ENSMUST00000179939 ENSMUST00000177974 ENSMUST00000031423 ENSMUST00000110299 ENSMUST00000112185 ENSMUST00000112183 ENSMUST00000043283 ENSMUST00000086564 ENSMUST00000155908 ENSMUST00000178440 ENSMUST00000051169 ENSMUST00000128901 ENSMUST00000036376 ENSMUST00000100387 ENSMUST00000071881 ENSMUST00000050205 ENSMUST00000110827 ENSMUST00000110826

Gene Information

Description Not Available

Gene Symbol:   Nudt1
Gene Name:   Nudix (nucleoside diphosphate linked moiety X)-type motif 1
Aliases:   Mth1
RefSeq:   NC_000071.6 NT_039316.8
Ensembl:   ENSMUSG00000036639
Entrez:   17766
Chromosome Mapping:   5|5 G1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.680000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download