PrimePCR™ SYBR® Green Assay: AC147549.4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043943

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041727
Assay Design:   exonic
Chromosome Location:   Y:10533802-10533940question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000179442 ENSMUST00000180125 ENSMUST00000178190 ENSMUST00000179746 ENSMUST00000180228 ENSMUST00000178175 ENSMUST00000178528 ENSMUST00000178470 ENSMUST00000178596 ENSMUST00000180160 ENSMUST00000178917 ENSMUST00000179650 ENSMUST00000178580 ENSMUST00000179249 ENSMUST00000179378 ENSMUST00000177863 ENSMUST00000178560 ENSMUST00000180028 ENSMUST00000178739 ENSMUST00000180330 ENSMUST00000180366 ENSMUST00000177885 ENSMUST00000179902 ENSMUST00000179653 ENSMUST00000178334 ENSMUST00000180192 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   AC147549.4
Gene Name:   Ssty1 family member
Aliases:   Not Available
RefSeq:   NC_000087.7 NT_187040.1
Ensembl:   ENSMUSG00000095950
Entrez:   382131
UniGene:   Not Available
Chromosome Mapping:   Y A1|Y

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.580000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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