PrimePCR™ SYBR® Green Assay: Luzp4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043663

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Luzp4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Luzp4, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041447
Assay Design:   exonic
Chromosome Location:   X:148923760-148923970question
Amplicon Length:   181
Splice Variants Targeted:   ENSMUST00000112814 ENSMUST00000112804 ENSMUST00000112796 ENSMUST00000112788 ENSMUST00000101186 ENSMUST00000101190 ENSMUST00000112753 ENSMUST00000101185 ENSMUST00000163338 ENSMUST00000179348 ENSMUST00000177606 ENSMUST00000177554 ENSMUST00000179021 ENSMUST00000178169 ENSMUST00000101181 ENSMUST00000101180 ENSMUST00000112741 ENSMUST00000112740

Gene Information

Description Not Available

Gene Symbol:   Luzp4
Gene Name:   Leucine zipper protein 4
Aliases:   ENSMUSG00000072927
RefSeq:   NC_000086.7 NT_039718.8
Ensembl:   ENSMUSG00000079387
Entrez:   434865
Chromosome Mapping:   X F3|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 40.240000
Efficiency 83

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download