PrimePCR™ SYBR® Green Assay: Gapdh, Mouse

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0039581

List Price:  
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0027497
Assay Design:   Exonic
Chromosome Location:   6:125162278-125162382question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000104945 ENSMUST00000089534 ENSMUST00000082064 ENSMUST00000091621 ENSMUST00000109588 ENSMUST00000118875 ENSMUST00000117757 ENSMUST00000073605 ENSMUST00000050581 ENSMUST00000095717

Gene Information

Description Not Available

Gene Symbol:   Gapdh
Gene Name:   Glyceraldehyde-3-phosphate dehydrogenase
Aliases:   Gapd
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000057666
Entrez:   14433
UniGene:   Mm.304088
Chromosome Mapping   6 F2|6 56.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.390000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev C Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev D Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.