PrimePCR™ SYBR® Green Assay: ALKBH6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041149

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ALKBH6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ALKBH6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038427
Assay Design:   Intron-spanning
Chromosome Location:   19:36502320-36503983question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000576741 ENST00000572344 ENST00000273371 ENST00000330817 ENST00000444914 ENST00000314669 ENST00000545060 ENST00000537681 ENST00000378875 ENST00000485128 ENST00000252984 ENST00000475223 ENST00000433672

Gene Information

Description Not Available

Gene Symbol:   ALKBH6
Gene Name:   alkB, alkylation repair homolog 6 (E. coli)
Aliases:   ABH6, MGC15677
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000239382
Entrez:   84964
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.170000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download