PrimePCR™ SYBR® Green Assay: CXorf40A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041009

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CXorf40A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CXorf40A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038287
Assay Design:   Intron-spanning
Chromosome Location:   X:148627341-148628345question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000450602 ENST00000441248 ENST00000393985 ENST00000423421 ENST00000423540 ENST00000434353 ENST00000514208 ENST00000428236 ENST00000422892 ENST00000359293 ENST00000462691 ENST00000370406 ENST00000355203 ENST00000370404 ENST00000595814 ENST00000601066 ENST00000597294 ENST00000598640 ENST00000596374 ENST00000597532 ENST00000594483 ENST00000601621 ENST00000598998 ENST00000593915 ENST00000595555 ENST00000601122 ENST00000596222 ENST00000600111

Gene Information

Description Not Available

Gene Symbol:   CXorf40A
Gene Name:   chromosome X open reading frame 40A
Aliases:   CXorf40, EOLA1, FLJ52212
RefSeq:   NC_000023.10 NT_011681.16
Ensembl:   ENSG00000197620
Entrez:   91966
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.550000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download