PrimePCR™ SYBR® Green Assay: TSGA14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040697

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TSGA14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TSGA14, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037974
Assay Design:   Intron-spanning
Chromosome Location:   7:130044483-130052037question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000264042 ENST00000223208 ENST00000343969 ENST00000492389 ENST00000472739 ENST00000477003 ENST00000475282 ENST00000541543 ENST00000264036 ENST00000392814 ENST00000368773 ENST00000580443 ENST00000583790 ENST00000577507 ENST00000578060 ENST00000579704 ENST00000582998 ENST00000582105

Gene Information

Description Not Available

Gene Symbol:   TSGA14
Gene Name:   testis specific, 14
Aliases:   Cep41, DKFZp762H1311
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000106477
Entrez:   95681
Chromosome Mapping:   7q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.550000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download