PrimePCR™ SYBR® Green Assay: LY75-CD302, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040244

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LY75-CD302, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LY75-CD302, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037521
Assay Design:   Intron-spanning
Chromosome Location:   2:160750486-160755308question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000551177 ENST00000546892 ENST00000455920 ENST00000356924 ENST00000298816 ENST00000263636 ENST00000554112 ENST00000553424 ENST00000504764 ENST00000505052 ENST00000400274 ENST00000417564 ENST00000318711 ENST00000253083 ENST00000389243 ENST00000540800 ENST00000295713 ENST00000414359 ENST00000414007 ENST00000419187 ENST00000368518 ENST00000368516 ENST00000305097 ENST00000295709 ENST00000392105 ENST00000440309 ENST00000392106 ENST00000361170 ENST00000583088 ENST00000306601 ENST00000366471 ENST

Gene Information

Description Not Available

Gene Symbol:   LY75-CD302
Gene Name:   LY75-CD302 readthrough
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000248672
Entrez:   100526664
Chromosome Mapping:   2q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download