PrimePCR™ SYBR® Green Assay: PGBD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040229

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PGBD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PGBD3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037506
Assay Design:   Intron-spanning
Chromosome Location:   10:50725072-50732172question
Amplicon Length:   160
Splice Variants Targeted:   ENST00000546361 ENST00000198939 ENST00000560626 ENST00000558305 ENST00000312493 ENST00000273666 ENST00000225873 ENST00000585380 ENST00000374127 ENST00000447839 ENST00000515869

Gene Information

The piggyBac family of proteins found in diverse animals are transposases related to the transposase of the canonical piggyBac transposon from the moth Trichoplusia ni. This family also includes genes in several genomes including human that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel with no obvious relationship to other transposases or other known protein families. This gene overlaps with the ERCC6 gene on chromosome 10 and pseudogenes of this locus have been found on chromosomes 4 5 and 12. [provided by RefSeq Jul 2008]

Gene Symbol:   PGBD3
Gene Name:   piggyBac transposable element derived 3
Aliases:   FLJ90201
RefSeq:   NC_000010.10 NG_009442.1 NT_030059.13
Ensembl:   ENSG00000243251
Entrez:   267004
Chromosome Mapping:   10q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.350000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download