PrimePCR™ SYBR® Green Assay: MAGED2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039105

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAGED2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAGED2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0036381
Assay Design:   Intron-spanning
Chromosome Location:   X:54839921-54841143question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000375068 ENST00000347546 ENST00000375058 ENST00000375060 ENST00000375053 ENST00000343474 ENST00000375062 ENST00000218439 ENST00000396224

Gene Information

This gene is a member of the MAGED gene family. While the MAGEA and MAGEB genes are silent in normal tissues with the exception of testis and placenta the MAGED genes are expressed ubiquitously. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2 a hot spot for X-linked mental retardation (XLMR). Multiple alternatively spliced transcript variants have been found for this gene however the full length nature of some variants has not been defined. [provided by RefSeq Jul 2008]

Gene Symbol:   MAGED2
Gene Name:   melanoma antigen family D, 2
Aliases:   11B6, BCG1, HCA10, JCL-1, MAGE-D2, MAGED, MGC8386
RefSeq:   NC_000023.10 NT_011630.14 NG_012844.1
Ensembl:   ENSG00000102316
Entrez:   10916
Chromosome Mapping:   Xp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.480000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download