PrimePCR™ SYBR® Green Assay: LIMA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023087
Assay Design:   Intron-spanning
Chromosome Location:   12:50571767-50575722question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000552491 ENST00000547825 ENST00000552823 ENST00000394943 ENST00000341247 ENST00000552783 ENST00000552909 ENST00000420992

Gene Information

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene and expression of some of the variants maybe independently regulated. [provided by RefSeq Aug 2011]

Gene Symbol:   LIMA1
Gene Name:   LIM domain and actin binding 1
Aliases:   EPLIN, FLJ38853, MGC131726, SREBP3
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000050405
Entrez:   51474
UniGene:   Hs.525419
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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