PrimePCR™ SYBR® Green Assay: TNIP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033415

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TNIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TNIP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022406
Assay Design:   Intron-spanning
Chromosome Location:   5:150439921-150441745question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000520931 ENST00000315050 ENST00000523338 ENST00000522226 ENST00000521591 ENST00000518977 ENST00000524280 ENST00000523200 ENST00000522100 ENST00000520695 ENST00000521001 ENST00000544828 ENST00000389378 ENST00000539213 ENST00000417127 ENST00000545840

Gene Information

This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis rheumatoid arthritis and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:   TNIP1
Gene Name:   TNFAIP3 interacting protein 1
Aliases:   ABIN-1, KIAA0113, NAF1, VAN
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000145901
Entrez:   10318
Chromosome Mapping:   5q32-q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.110000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download