PrimePCR™ SYBR® Green Assay: LAMB3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033190

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LAMB3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LAMB3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021796
Assay Design:   Intron-spanning
Chromosome Location:   1:209811908-209823352question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000356082 ENST00000367030 ENST00000415782 ENST00000391911

Gene Information

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin which together with an alpha and a gamma subunit forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type and generalized atrophic benign epidermolysis bullosa diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LAMB3
Gene Name:   laminin, beta 3
Aliases:   BM600-125KDA, FLJ99565, LAM5, LAMNB1
RefSeq:   NC_000001.10 NG_007116.1 NT_167186.1
Ensembl:   ENSG00000196878
Entrez:   3914
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.960000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download