PrimePCR™ SYBR® Green Assay: ASB7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021716
Assay Design:   Intron-spanning
Chromosome Location:   15:101152547-101169698question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000332783 ENST00000343276

Gene Information

The protein encoded by this gene belongs to a family of ankyrin repeat proteins that along with four other protein families contains a C-terminal SOCS box motif. Growing evidence suggests that the SOCS box acts as a bridge between specific substrate-binding domains and the more generic proteins that comprise a large family of E3 ubiquitin protein ligases. In this way SOCS box containing proteins may regulate protein turnover by targeting proteins for polyubiquination and therefore for proteasome-mediated degradation. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   ASB7
Gene Name:   ankyrin repeat and SOCS box containing 7
Aliases:   FLJ22551, FLJ25192
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000183475
Entrez:   140460
UniGene:   Hs.31845
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.950000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download