PrimePCR™ SYBR® Green Assay: LTN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031875

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LTN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LTN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018473
Assay Design:   Intron-spanning
Chromosome Location:   21:30343699-30353519question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000389194 ENST00000389195 ENST00000361371 ENST00000446611

Gene Information

Like most RING finger proteins LTN1 functions as an E3 ubiquitin ligase (Chu et al. 2009 [PubMed 19196968]).[supplied by OMIM Nov 2010]

Gene Symbol:   LTN1
Gene Name:   listerin E3 ubiquitin protein ligase 1
Aliases:   C21orf10, C21orf98, FLJ11053, KIAA0714, RNF160, ZNF294
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000198862
Entrez:   26046
Chromosome Mapping:   21q22.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.860000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download