PrimePCR™ SYBR® Green Assay: TSPAN12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031803

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TSPAN12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TSPAN12, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018354
Assay Design:   Intron-spanning
Chromosome Location:   7:120480087-120496818question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000222747 ENST00000415871 ENST00000441017 ENST00000433758 ENST00000424710 ENST00000430985

Gene Information

The protein encoded by this gene is a member of the transmembrane 4 superfamily also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development activation growth and motility. [provided by RefSeq Jul 2008]

Gene Symbol:   TSPAN12
Gene Name:   tetraspanin 12
Aliases:   EVR5, NET-2, NET2, TM4SF12
RefSeq:   NC_000007.13 NG_023203.1 NT_007933.15
Ensembl:   ENSG00000106025
Entrez:   23554
Chromosome Mapping:   7q31.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.010000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download