PrimePCR™ SYBR® Green Assay: BCAR3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031719

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018205
Assay Design:   Intron-spanning
Chromosome Location:   1:94027863-94032874question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000370247 ENST00000260502 ENST00000370244 ENST00000370243 ENST00000539242

Gene Information

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain a hall mark of cellular tyrosine kinase signaling molecules and is partly homologous to the cell division cycle protein CDC48. [provided by RefSeq Jul 2008]

Gene Symbol:   BCAR3
Gene Name:   breast cancer anti-estrogen resistance 3
Aliases:   KIAA0554, NSP2, SH2D3B
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000137936
Entrez:   8412
UniGene:   Hs.36958
Chromosome Mapping:   1p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.050000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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