PrimePCR™ SYBR® Green Assay: GUCA1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030490

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GUCA1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GUCA1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016277
Assay Design:   Intron-spanning
Chromosome Location:   6:42141501-42146064question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000418175 ENST00000053469 ENST00000394237 ENST00000372958 ENST00000541991 ENST00000372965

Gene Information

This gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene guanylyl cyclase activating protein 1 (GCAP1) mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in this gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. Mutations in this gene prohibit the inactivation of RetGC1 at high Ca(2+) concentrations; causing the constitutive activation of RetGC1 and presumably increased cell death. This gene is expressed in retina and spermatagonia. [provided by RefSeq Feb 2009]

Gene Symbol:   GUCA1A
Gene Name:   guanylate cyclase activator 1A (retina)
Aliases:   C6orf131, COD3, CORD14, GCAP, GCAP1, GUCA, GUCA1, dJ139D8.6
RefSeq:   NC_000006.11 NG_009938.1 NT_007592.15
Ensembl:   ENSG00000048545
Entrez:   2978
Chromosome Mapping:   6p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download