This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0030127
PrimePCR™ PreAmp for SYBR® Green Assay: STX1B, Human
PrimePCR™ Template for SYBR® Green Assay: STX1B, Human
Syntaxins are cellular receptors for transport vesicles (see MIM 603765). One of these proteins designated syntaxin 1B (STX1B) is directly implicated in the process of calcium-dependent synaptic transmission in rat brain (Smirnova et al. 1993 [PubMed 8105537]). The expression of this protein is transiently induced by long-term potentiation of synaptic responses in the rat hippocampus. The protein may play an important role in the excitatory pathway of synaptic transmission which is known to be implicated in several neurologic diseases.[supplied by OMIM Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.