PrimePCR™ SYBR® Green Assay: SLC25A31, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030092

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC25A31, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC25A31, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015681
Assay Design:   Intron-spanning
Chromosome Location:   4:128651854-128665927question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000281154

Gene Information

Mitochondrial ADP/ATP carriers such as SLC25A31 are nuclear-coded mitochondrial proteins that catalyze the exchange of ATP generated in mitochondria by ATP synthase (see MIM 108729) against ADP produced in cytosol by most energy-consuming reactions (Dolce et al. 2005 [PubMed 15670820]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC25A31
Gene Name:   solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
Aliases:   AAC4, ANT4, DKFZp434N1235, SFEC35kDa
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000151475
Entrez:   83447
Chromosome Mapping:   4q28.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.710000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download